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JoVE Journal Biochemistry
Analyse der Proteinfaltung, Transport und Abbau in lebenden Zellen durch radioaktive Puls Chase
 
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Analyse der Proteinfaltung, Transport und Abbau in lebenden Zellen durch radioaktive Puls Chase

Article DOI: 10.3791/58952-v • 08:59 min • February 12th, 2019
February 12th, 2019 •
1,2, 1, 1, 1, 1, 1
1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

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Hier beschreiben wir ein Protokoll für eine allgemeine Puls-Jagd-Methode, die die kinetische Analyse der Faltung, Transport und Abbau von Proteinen in lebenden Zellen einzuhaltenden erlaubt.

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Biochemie Ausgabe 144 Protein Falten enzymatische SDS-PAGE Immunopräzipitation Puls zu jagen conformational Analyse Kinetik
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