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Analyse van de vouwing van eiwitten, vervoer en afbraak in levende cellen door radioactieve Pulse Chase

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

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Analyse van de vouwing van eiwitten, vervoer en afbraak in levende cellen door radioactieve Pulse Chase

10,812 Views

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:

10.3791/58952-v

•

08:59 min

February 12, 2019

•

2 Views

Nicholas McCaul², Hui Ying Yeoh, Guus van Zadelhoff, Naomi Lodder, Bertrand Kleizen, Ineke Braakman

¹Cellular Protein Chemistry,Utrecht University, ²Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Tags

Protein Folding Protein Transport Protein Degradation Radioactive Pulse Chase 35S-labeled Methionine And Cysteine Protein Biosynthesis Co- And Post-translational Modifications Disulfide Bond Formation Acetylation Cell Culture Transfection Starvation Medium Pulse Solution Chase Medium Stop Buffer

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