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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
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Bioquímica
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JoVE Journal Bioquímica
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:
10.3791/58952-v

•

08:59 min
February 12, 2019

•

2 Views
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1Cellular Protein Chemistry,Utrecht University, 2Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Tags

Protein FoldingProtein TransportProtein DegradationRadioactive Pulse Chase35S-labeled Methionine And CysteineProtein BiosynthesisCo- And Post-translational ModificationsDisulfide Bond FormationAcetylationCell CultureTransfectionStarvation MediumPulse SolutionChase MediumStop Buffer

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McCaul, N., Yeoh, H. Y., van Zadelhoff, G., Lodder, N., Kleizen, B., Braakman, I. Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase. J. Vis. Exp. (144), e58952, doi:10.3791/58952 (2019).

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