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Imunologia e Infecção

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化学抑制剂库筛选 t 细胞受体信号的中介识别

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

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化学抑制剂库筛选 t 细胞受体信号的中介识别

DOI:

10.3791/58946-v

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08:49 min

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January 22, 2019

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Elijah W. Chen*¹, Chyan Ying Ke*^2,3, Joanna Brzostek*¹, Nicholas R. J. Gascoigne, Vasily Rybakin⁴

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, ²Singapore Immunology Network,A*STAR, ³Curiox Biosystems, ⁴Department of Immunobiology, Rega Institute for Medical Research,Katholieke Universiteit (KU) Leuven

Capítulos

00:04Título
01:15Kinase Inhibitor Library Screening (Centrifuge-independent Assay)
02:15Stimulation of Thymocytes Using Anti-CD3/CD28 Beads
03:52Staining of Surface Antigens and Fixation of Cells
05:09Intracellular Staining for Active Caspase 3
06:05Results: Flow-Cytometry-Based Screening of Modulators of T-cell Receptor Signaling and T-cell Activation
07:59Conclusion

Summary

Tadução automática

本文采用基于流式细胞仪的检测方法筛选化学抑制剂文库, 用于鉴定影响 t 细胞受体信号转导的抑制剂及其靶点。此处描述的方法也可以扩展为高通量筛选。

Tags

Keywords: T-cell Receptor Signaling Chemical Inhibitor Libraries High-throughput Assay TCR Signaling Mediators Immune Diseases Thymocytes Kinase Inhibitors Anti-CD3/CD28 Beads Apoptosis

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