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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

8,799 Views

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08:49 min

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January 22, 2019

DOI:

10.3791/58946-v

DOI:
10.3791/58946-v

•

08:49 min
January 22, 2019

•

3 Views
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1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Singapore Immunology Network,A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research,Katholieke Universiteit (KU) Leuven

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This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.

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Chen, E. W., Ke, C. Y., Brzostek, J., Gascoigne, N. R. J., Rybakin, V. Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries. J. Vis. Exp. (143), e58946, doi:10.3791/58946 (2019).

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