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活性型のニコチンのレーザーフラッシュ・フォトリシス法によるマウス脳切片でのニコチン性アセチルコリン受容体の機能を探る

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

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活性型のニコチンのレーザーフラッシュ・フォトリシス法によるマウス脳切片でのニコチン性アセチルコリン受容体の機能を探る

8,925 Views

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10:48 min

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January 25, 2019

DOI:

10.3791/58873-v

DOI:

10.3791/58873-v

•

10:48 min

January 25, 2019

•

1 Views

Matthew C. Arvin, David L. Wokosin, Sambashiva Banala, Luke D. Lavis, Ryan M. Drenan

¹Department of Pharmacology,Northwestern University Feinberg School of Medicine, ²Department of Physiology,Northwestern University Feinberg School of Medicine, ³Janelia Research Campus,Howard Hughes Medical Institute

Tags

Laser Flash Photolysis Photoactivatable Nicotine Nicotinic Acetylcholine Receptors Mouse Brain Slices Multi-photon Laser-scanning Microscopy Spatiotemporal Control Cholinergic Synaptic Transmission Volume Transmission Patch Clamp Pressure Ejection Bath Application Perfusion System

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