Rivista
/
Neuroscienze
/
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

8,925 Views

•

10:48 min

•

January 25, 2019

DOI:

10.3791/58873-v

DOI:
10.3791/58873-v

•

10:48 min
January 25, 2019

•

1 Views
, , , ,
1Department of Pharmacology,Northwestern University Feinberg School of Medicine, 2Department of Physiology,Northwestern University Feinberg School of Medicine, 3Janelia Research Campus,Howard Hughes Medical Institute

Tags

Laser Flash PhotolysisPhotoactivatable NicotineNicotinic Acetylcholine ReceptorsMouse Brain SlicesMulti-photon Laser-scanning MicroscopySpatiotemporal ControlCholinergic Synaptic TransmissionVolume TransmissionPatch ClampPressure EjectionBath ApplicationPerfusion System

Read Article

Cite this Article

Arvin, M. C., Wokosin, D. L., Banala, S., Lavis, L. D., Drenan, R. M. Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine. J. Vis. Exp. (143), e58873, doi:10.3791/58873 (2019).

Download .ris file

Copy

Share Video

.

Copy