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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
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JoVE Journal Neurociência
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

DOI:
10.3791/58840-v

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07:18 min

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January 16, 2019

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,
1Department of Molecular Physiology and Biophysics,University of Fukui Faculty of Medical Sciences

Capítulos

  • 00:04Título
  • 00:58Liposomes and Pipettes Preparation
  • 02:41Electrophysiological Measurement
  • 05:49Results: Detach-attach of Monolayers and Responses of Channels
  • 06:37Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol for the formation of lipid bilayers using a contact bubble bilayer method. A water bubble is blown into an organic solvent, whereby a monolayer is formed at the water-oil interface. Two pipettes are manipulated to dock the bubbles to form a bilayer.

Tags

Keywords: Lipid BilayerContact Bubble BilayerPatch-clampingPlanar Lipid BilayerMembrane VibrationSingle Channel CurrentPhospholipid SuspensionProteoliposomesIon Channel ProteinsGlass PipettesMicro ForgeSiliconizing ReagentInverted Microscope

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