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建立与艾滋病毒相关的肝脏发病机制的双人性化TK-NOG小鼠模型

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Please note that all translations are automatically generated. Click here for the English version.

建立与艾滋病毒相关的肝脏发病机制的双人性化TK-NOG小鼠模型

DOI:

10.3791/58645-v

•

00:10 min

•

September 11, 2019

•

Raghubendra Singh Dagur, Weimin Wang, Edward Makarov, Yimin Sun, Larisa Y. Poluektova

¹Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, ²Department of Pathology and Microbiology,University of Nebraska Medical Center

Capítulos

00:04Título
00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
04:25Preparation of Human Hepatocytes for Transplantation
05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
09:25Conclusion

Summary

Tadução automática

该协议提供了一种可靠的方法，建立人类免疫系统和肝细胞的人性化小鼠。通过注射人肝细胞和CD34实现的双重组免疫缺陷小鼠^–造血干细胞易受人体免疫缺陷病毒-1感染和重述肝损伤的影响，如艾滋病毒感染者。

Tags

Keywords: HIV Liver Pathogenesis Humanized Mouse Model Hematopoietic Stem Cells Hepatocytes Umbilical Cord Blood CD34 Positive Cells Cell Separation Magnetic Activated Cell Sorting (MACS)Cell Counting Cell Culture Transplantation

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