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Grootschalige topdown Proteomics met behulp van capillaire Zone elektroforese Tandem massaspectrometrie
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Grootschalige topdown Proteomics met behulp van capillaire Zone elektroforese Tandem massaspectrometrie

9,107 Views

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10:05 min

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October 24, 2018

DOI:

10.3791/58644-v

DOI:
10.3791/58644-v

•

10:05 min
October 24, 2018

•

4 Views
, , , , ,
1Department of Chemistry,Michigan State University, 2Department of BioHealth Informatics,Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics,Indiana University School of Medicine

Summary

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Een gedetailleerd protocol wordt beschreven voor de scheiding, identificatie en karakterisering van proteoforms in EiwitSteekproeven met behulp van capillaire zone elektroforese-electrospray ionisatie-tandem massaspectrometrie (CZE-ESI-MS/MS). Het protocol kan worden gebruikt voor de hoge-resolutie karakterisatie van proteoforms in simple EiwitSteekproeven en de grootschalige identificatie van proteoforms in complexe Proteoom monsters.

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Cite this Article

McCool, E. N., Lubeckyj, R., Shen, X., Kou, Q., Liu, X., Sun, L. Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry. J. Vis. Exp. (140), e58644, doi:10.3791/58644 (2018).

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