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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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化学
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JoVE Journal 化学
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

9,120 Views

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10:05 min

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October 24, 2018

DOI:

10.3791/58644-v

DOI:
10.3791/58644-v

•

10:05 min
October 24, 2018

•

4 Views
, , , , ,
1化学科,Michigan State University, 2Department of BioHealth Informatics,Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics,Indiana University School of Medicine

概要

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A detailed protocol is described for the separation, identification, and characterization of proteoforms in protein samples using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS). The protocol can be used for the high-resolution characterization of proteoforms in simple protein samples and the large-scale identification of proteoforms in complex proteome samples.

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Cite this Article

McCool, E. N., Lubeckyj, R., Shen, X., Kou, Q., Liu, X., Sun, L. Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry. J. Vis. Exp. (140), e58644, doi:10.3791/58644 (2018).

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