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利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学
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JoVE Journal Química
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学

9,107 Views

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10:05 min

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October 24, 2018

DOI:

10.3791/58644-v

DOI:
10.3791/58644-v

•

10:05 min
October 24, 2018

•

4 Views
, , , , ,
1Department of Chemistry,Michigan State University, 2Department of BioHealth Informatics,Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics,Indiana University School of Medicine

Summary

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通过毛细管区电泳-电喷雾-串联质谱 (CZE-proteoforms), 描述了蛋白质样品中的分离、鉴定和表征的详细协议。该协议可用于简单蛋白样品中 proteoforms 的高分辨率表征和复杂蛋白质组样本中 proteoforms 的大尺度鉴定。

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ProteomicsCapillary Zone ElectrophoresisMass SpectrometryProtein IsoformsProtein Post-translational ModificationsSample PreparationCapillary CoatingCapillary EtchingCZE System Setup

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Cite this Article

McCool, E. N., Lubeckyj, R., Shen, X., Kou, Q., Liu, X., Sun, L. Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry. J. Vis. Exp. (140), e58644, doi:10.3791/58644 (2018).

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