/

/

大鼠甲基 Seq 平台识别与应力接触相关的表观变化

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Please note that all translations are automatically generated. Click here for the English version.

大鼠甲基 Seq 平台识别与应力接触相关的表观变化

DOI:

10.3791/58617-v

•

09:06 min

•

October 24, 2018

•

Jenny L. Carey, Olivia H. Cox, Fayaz Seifuddin, Leonard Marque, Kellie L.K. Tamashiro, Peter P. Zandi³, Gary S. Wand², Richard S. Lee

¹Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, ²Department of Medicine,Johns Hopkins School of Medicine, ³Department of Mental Health,Johns Hopkins School of Public Health

Capítulos

00:04Título
01:04Sample Preparation
01:54Hybridization
04:16Bisulfite Conversion
05:29Indexing
06:06Validation by Bisulfite Pyrosequencing
07:18Results: Stress-associated DNA Methylation Changes are verified by Bisulfite Pyrosequencing
08:03Conclusion

Summary

Tadução automática

在这里, 我们描述了同道平台甲基 Seq 的协议和实现, 使用老鼠模型来识别与慢性应力暴露相关的表观变化。结果表明, 大鼠甲基序列平台能够检测大鼠应力暴露引起的甲基化差异。

Tags

Keywords: Rat Methyl-Seq DNA Methylation Epigenomics Environmental Factors Physiological Processes Stress Exposure Sequencing-based Tool Array-based Platforms DNA Shearing Magnetic Beads DNA-binding Adapter Ligation Methyl-Seq Block Mix Capture Library Hybridization Mix Streptavidin Magnetic Beads

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

从多个小鼠组织分离中间丝状蛋白以研究与老化相关的翻译后修饰

利用综合沉淀富集系统识别目标蛋白的内源性后向转化剖面

基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

从高梁叶组织中分离出希石，用于自上而下质量光谱分析潜在的表观遗传标记

具有可移动XY阶段的平台孵化器：实现蛋白质细胞内快速光化学氧化的新平台

细胞膜亲和色谱柱鉴定与固定化托肌球蛋白激酶受体B相互作用的特化植物代谢物

DetectSyn：一种快速、无偏倚的荧光方法，用于检测突触密度的变化

一种基于质谱的磷酸化蛋白磷酸酶及其相互作用物鉴定方法

一种分析脑血凝块细胞成分的综合方法

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Read Article