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A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

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09:06 min

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October 24, 2018

DOI:

10.3791/58617-v

DOI:
10.3791/58617-v

•

09:06 min
October 24, 2018

•

1 Views
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1Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, 2Department of Medicine,Johns Hopkins School of Medicine, 3Department of Mental Health,Johns Hopkins School of Public Health

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Here, we describe the protocol and implementation of Methyl-Seq, an epigenomic platform, using a rat model to identify epigenetic changes associated with chronic stress exposure. Results demonstrate that the rat Methyl-Seq platform is capable of detecting methylation differences that arise from stress exposure in rats.

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Rat Methyl-SeqDNA MethylationEpigenomicsEnvironmental FactorsPhysiological ProcessesStress ExposureSequencing-based ToolArray-based PlatformsDNA ShearingMagnetic BeadsDNA-bindingAdapter LigationMethyl-Seq Block MixCapture Library Hybridization MixStreptavidin Magnetic Beads

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Carey, J. L., Cox, O. H., Seifuddin, F., Marque, L., Tamashiro, K. L., Zandi, P. P., Wand, G. S., Lee, R. S. A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure. J. Vis. Exp. (140), e58617, doi:10.3791/58617 (2018).

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