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利用网格和收集荧光蛋白陶瓷的结构解
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
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利用网格和收集荧光蛋白陶瓷的结构解

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06:42 min

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March 19, 2019

DOI:

10.3791/58594-v

DOI:
10.3791/58594-v

•

06:42 min
March 19, 2019

•

1 Views
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1European Synchrotron Radiation Facility,Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

Summary

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我们介绍了使用网格德德收集协议来获得一个完整的衍射数据集, 用于随后的结构测定, 由从荧光蛋白 Cerulean 的许多小晶体中收集的部分衍射数据集组成。

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Cite this Article

Hutin, S., Santoni, G., Zander, U., Foos, N., Aumonier, S., Gotthard, G., Royant, A., Mueller-Dieckmann, C., Leonard, G. Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect. J. Vis. Exp. (145), e58594, doi:10.3791/58594 (2019).

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