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Celle-baserede analyse at studere antistof-medieret Tau Clearance af mikroglia
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Celle-baserede analyse at studere antistof-medieret Tau Clearance af mikroglia

DOI:
10.3791/58576-v

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07:18 min

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November 09, 2018

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, , , , ,
1Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis,Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center,Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Capítulos

  • 00:04Título
  • 00:37Uptake Assay with Fluorescence-activated Cell Sorting (FACS) Read-out
  • 04:33FACS Analysis
  • 05:41Results: Antibody-mediated Increase of Tau Uptake in BV-2 Cells
  • 06:45Conclusion

Summary

Tadução automática

Tadução automática

Her beskriver vi en celle-baseret analyse for at vurdere kvantitativt tau optagelse af mikroglia med formålet at skabe et testpræparat værktøj til bedre karakterisere virkningsmekanisme af anti-tau antistoffer.

Tags

Keywords: Cell-based AssayAntibody-mediated Tau ClearanceMicrogliaAlzheimer’s DiseasePH Dye-Tau AggregatesAnti-Tau AntibodiesBV-2 CellsCell CultureTrypsin EDTACentrifugation

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