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Cryosectioning simultánea de varios cerebros de roedores
1Department of Child Health,University of Arizona College of Medicine, 2Department of Biological Sciences,University of Bath, 3BARROW Neurological Institute at Phoenix Children’s Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences,University of Arizona College of Medicine
Capítulos
- 00:04Título
- 00:44Cryoprotection of Perfused Brains and Brain Freezing
- 03:18Preparing the Megabrain for Sectioning on the Cryostat
- 04:14Cryosectioning the Megabrain
- 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
- 05:54Conclusion
Aquí, presentamos un protocolo de congelación y sección de tejido cerebral de varios animales como una alternativa de ahorro de tiempo a procesar solo cerebros. Esto reduce la variabilidad de coloración durante la inmunohistoquímica y reduce la proyección de imagen y tiempo cryosectioning.
