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급성 척수 조각 Substantia Gelatinosa 신경에 있는 전체 셀 패치 클램프 기록에 대 한 준비

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

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급성 척수 조각 Substantia Gelatinosa 신경에 있는 전체 셀 패치 클램프 기록에 대 한 준비

13,453 Views

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08:30 min

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January 18, 2019

DOI:

10.3791/58479-v

DOI:

10.3791/58479-v

•

08:30 min

January 18, 2019

•

10 Views

Mengye Zhu, Daying Zhang, Sicong Peng, Nana Liu, Jing Wu, Haixia Kuang, Tao Liu³

¹Department of Pain,First Affiliated Hospital of Nanchang University, ²Department of Pediatrics,First Affiliated Hospital of Nanchang University, ³Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Tags

Acute Spinal Cord Slices Whole-cell Patch-clamp Recording Substantia Gelatinosa Neurons Sensory Transmission Nociceptive Regulation Chronic Pain Agar Block Sucrose-based ACSF Transcardial Perfusion Spinal Cord Extraction Lumbosacral Enlargement Meninges Removal Pia Arachnoid Membrane Ventral/dorsal Roots Transverse Slices Parasagittal Slices Vibratome

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