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Forberedelse af akut rygmarven skiver til hele-celle Patch-klemme optagelse i Substantia Gelatinosa neuroner
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Neurociência
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JoVE Journal Neurociência
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Forberedelse af akut rygmarven skiver til hele-celle Patch-klemme optagelse i Substantia Gelatinosa neuroner

13,453 Views

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08:30 min

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January 18, 2019

DOI:

10.3791/58479-v

DOI:
10.3791/58479-v

•

08:30 min
January 18, 2019

•

10 Views
, , , , , ,
1Department of Pain,First Affiliated Hospital of Nanchang University, 2Department of Pediatrics,First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Tags

Acute Spinal Cord SlicesWhole-cell Patch-clamp RecordingSubstantia Gelatinosa NeuronsSensory TransmissionNociceptive RegulationChronic PainAgar BlockSucrose-based ACSFTranscardial PerfusionSpinal Cord ExtractionLumbosacral EnlargementMeninges RemovalPia Arachnoid MembraneVentral/dorsal RootsTransverse SlicesParasagittal SlicesVibratome

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Zhu, M., Zhang, D., Peng, S., Liu, N., Wu, J., Kuang, H., Liu, T. Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons. J. Vis. Exp. (143), e58479, doi:10.3791/58479 (2019).

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