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Immunisering af voksen zebrafisk for præklinisk Screening af DNA-baserede vacciner
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
DOI:

05:39 min

October 30, 2018

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Capítulos

  • 00:04Título
  • 00:35Pulling the Microinjection Needles
  • 01:10Filling the Micropipette Needles
  • 01:49Setting the Microinjector and Electroporator for Immunization
  • 02:40Injection of the DNA Vaccine and Electroporation and Visualization of Antigen Expression
  • 04:04Results: Danio rerio Injected with DNA-based Vaccine
  • 05:04Conclusion

Summary

Tadução automática

Her beskriver vi en protokol for immunisering af de voksne zebrafisk (Danio rerio) med en DNA-baseret vaccine og demonstrere validering af en vellykket vaccination begivenhed. Denne metode er velegnet til præklinisk screening af vaccine kandidater i forskellige infektion modeller.

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