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1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer,Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering,Imperial College London, 3Department of Bioengineering,Imperial College London, 4Department of Life Sciences,Imperial College London, 5Department of Anaesthetics,Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine,Medical Directorate Joint Force Command
Capítulos
- 00:04Título
- 00:38Preparation of Hippocampal Organotypic Slices for the Experimental Blast TBI Protocol
- 02:39Submersion and Transport of Tissue Culture Inserts with Hippocampal Organotypic Slices
- 03:44Preparation of the Shock Tube and Hippocampal Organotypic Slice Shock Wave Exposure
- 07:05Results: Typical Shockwave and Resulting Injury in Organotypic Hippocampal Slice
- 08:11Conclusion
Questo articolo descrive un nuovo modello di ferita di cervello traumatica esplosione primaria. Un tubo di aria compressa guidato d'urto viene utilizzato per esporre in vitro culture hippocampal fetta di mouse per una singola onda d'urto. Si tratta di un protocollo semplice e rapido, generando una lesione del tessuto di cervello riproducibile con un throughput elevato.
