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A Novel In Vitro Model of Blast Traumatic Brain Injury
 

A Novel In Vitro Model of Blast Traumatic Brain Injury

Article DOI: 10.3791/58400-v • 08:59 min • December 21st, 2018
December 21st, 2018 •
1,2, 1, 2,3, 1, 4,5, 6, 1,2
1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer, Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering, Imperial College London, 3Department of Bioengineering, Imperial College London, 4Department of Life Sciences, Imperial College London, 5Department of Anaesthetics, Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine, Medical Directorate Joint Force Command

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This paper describes a novel model of primary blast traumatic brain injury. A compressed-air driven shock tube is used to expose in vitro mouse hippocampal slice cultures to a single shock wave. This is a simple and rapid protocol generating a reproducible brain tissue injury with a high throughput.

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Novel In Vitro Model Blast Traumatic Brain Injury Neuroprotective Drugs In Vivo Models Laboratory Instrument Shockwave Exposure High Throughput Protocol Reproducible Injury Six Well Plate Serum-free Experimental Medium Propidium Iodide Tissue Culture Inserts Organotypic Slices Growth Dishes Unique Identifier Incubation At 37 Degrees Celsius Imaging
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