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An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
JoVE Journal
Ambiente
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JoVE Journal Ambiente
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

DOI:
10.3791/58324-v

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09:33 min

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December 17, 2018

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, ,
1Department of Biology,University of Ottawa

Capítulos

  • 00:04Título
  • 00:36Preparation of the Biosensor for Anaerobic Exposure Assay
  • 04:34Exposure Assay
  • 07:34Results: Interpreting the Biosensors Fluorescent Signal
  • 08:35Conclusion

Summary

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Here, we present a protocol to use an anaerobic whole-cell microbial biosensor to evaluate how different environmental variables affect the bioavailability of Hg and Cd to bacteria in anoxic environments.

Tags

Anaerobic BiosensorMercury DetectionCadmium DetectionBiogeochemical CyclingReal-time BioavailabilityBacterial ViabilityAnaerobic GrowthAmpicillinLysogeny BrothAnaerobic ChamberBalch Tube

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