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环境科学
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An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
JoVE 杂志
环境科学
This content is Free Access.
JoVE 杂志 环境科学
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

DOI:
10.3791/58324-v

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09:33 min

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December 17, 2018

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, ,
1Department of Biology,University of Ottawa

Chapters

  • 00:04Title
  • 00:36Preparation of the Biosensor for Anaerobic Exposure Assay
  • 04:34Exposure Assay
  • 07:34Results: Interpreting the Biosensors Fluorescent Signal
  • 08:35Conclusion

Summary

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Here, we present a protocol to use an anaerobic whole-cell microbial biosensor to evaluate how different environmental variables affect the bioavailability of Hg and Cd to bacteria in anoxic environments.

Tags

Anaerobic BiosensorMercury DetectionCadmium DetectionBiogeochemical CyclingReal-time BioavailabilityBacterial ViabilityAnaerobic GrowthAmpicillinLysogeny BrothAnaerobic ChamberBalch Tube

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