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Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
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Neurociência
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JoVE Journal Neurociência
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

DOI:
10.3791/58171-v

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06:17 min

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September 01, 2018

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, ,
1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Capítulos

  • 00:04Título
  • 00:38Surgical Exposure of the L4 and L5 DRGs
  • 01:37DRG Injection and Electroporation
  • 02:23Sciatic Nerve Crush
  • 03:07DRG and Sciatic Nerve Harvest and Imaging
  • 04:23Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
  • 05:35Conclusion

Summary

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Electroporation is an effective approach to deliver genes of interests into cells. By applying this approach in vivo on the neurons of adult mouse dorsal root ganglion (DRG), we describe a model to study axon regeneration in vivo.

Tags

Keywords: In Vivo ElectroporationAdult Mouse Dorsal Root GanglionGene TransfectionAxon RegenerationSensory NeuronsSciatic Nerve CrushNerve InjuryGene OverexpressionGene Knockdown

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