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Dinamica proteomica e miRNA analisi di polisomi da Mouse cuore dopo Langendorff aspersione isolata
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Bioquímica
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JoVE Journal Bioquímica
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
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Dinamica proteomica e miRNA analisi di polisomi da Mouse cuore dopo Langendorff aspersione isolata

8,477 Views

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07:54 min

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August 29, 2018

DOI:

10.3791/58079-v

DOI:
10.3791/58079-v

•

07:54 min
August 29, 2018

•

1 Views
, , , , ,
1The Smidt Heart Institute,Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute,Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

Summary

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Qui presentiamo un protocollo per eseguire polisoma profilatura sul cuore isolato e perfuso del mouse. Descriviamo i metodi per aspersione di cuore, polisoma profiling e l'analisi delle frazioni polisoma rispetto al mRNA, Mirna e il proteoma polisoma.

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Cite this Article

Stastna, M., Thomas, A., Germano, J., Pourpirali, S., Van Eyk, J. E., Gottlieb, R. A. Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion. J. Vis. Exp. (138), e58079, doi:10.3791/58079 (2018).

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