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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
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Biochimie
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Journal JoVE Biochimie
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

8,485 Views

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07:54 min

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August 29, 2018

DOI:

10.3791/58079-v

DOI:
10.3791/58079-v

•

07:54 min
August 29, 2018

•

1 Views
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1The Smidt Heart Institute,Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute,Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

Summary

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Here we present a protocol to perform polysome profiling on the isolated perfused mouse heart. We describe methods for heart perfusion, polysome profiling, and analysis of the polysome fractions with respect to mRNAs, miRNAs, and the polysome proteome.

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Dynamic ProteomicsMiRNA AnalysisPolysomesLangendorff PerfusionCardiac Stress ResponseProtein SynthesisMRNA TranslationMicroRNAProtein ExtractionSucrose GradientUltracentrifugationFraction Collection

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Cite this Article

Stastna, M., Thomas, A., Germano, J., Pourpirali, S., Van Eyk, J. E., Gottlieb, R. A. Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion. J. Vis. Exp. (138), e58079, doi:10.3791/58079 (2018).

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