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Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

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Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析

8,477 Views

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07:54 min

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August 29, 2018

DOI:

10.3791/58079-v

DOI:

10.3791/58079-v

•

07:54 min

August 29, 2018

•

1 Views

Miroslava Stastna^1,2,3, Amandine Thomas¹, Juliana Germano¹, Somayeh Pourpirali¹, Jennifer E. Van Eyk^1,2, Roberta A. Gottlieb¹

¹The Smidt Heart Institute,Cedars-Sinai Medical Center, ²Advanced Clinical Biosystems Research Institute,Cedars-Sinai Medical Center, ³Institute of Analytical Chemistry of the Czech Academy of Sciences

Summary

在这里, 我们提出了一个协议, 执行 polysome 分析的孤立灌注小鼠心脏。我们描述的方法, 心脏灌注, polysome 分析, 并分析 polysome 分数的基因, miRNAs, polysome 蛋白质组。

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