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单液滴数字聚合酶链反应对热点地区突变的综合和同步检测
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08:23 min
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September 25, 2018
DOI:
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department,Institut Curie, PSL Research University, 2CNRS UMR144,Institut Curie, PSL Research University, 3Department of Medical Oncology,Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830,Institut Curie, PSL Research University
Summary
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在这里, 我们提出了一个协议, 以准确地量化一个目标区域的多基因变化, 在单一的反应使用下拉 ddPCR 和一个独特的对水解探针。
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Cite this Article
Decraene, C., Bortolini Silveira, A., Michel, M., Bidard, F., Pierga, J., Stern, M., Proudhon, C. Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions. J. Vis. Exp. (139), e58051, doi:10.3791/58051 (2018).
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