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用于膜蛋白结构分析和从头建模的小角中子散射实验中的对比匹配洗涤剂
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Bioquímica
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JoVE Journal Bioquímica
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
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用于膜蛋白结构分析和从头建模的小角中子散射实验中的对比匹配洗涤剂

12,106 Views

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10:27 min

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October 21, 2018

DOI:

10.3791/57901-v

DOI:
10.3791/57901-v

•

10:27 min
October 21, 2018

•

2 Views
, , , , ,
1Neutron Scattering Division,Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

Automatically generated

该协议演示了如何获得一个低分辨率的从头模型和洗涤剂-溶解膜蛋白在溶液中使用小角中子散射与洗涤剂的对比匹配的结构细节。

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Cite this Article

Oliver, R. C., Naing, S., Weiss, K. L., Pingali, S. V., Lieberman, R. L., Urban, V. S. Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling. J. Vis. Exp. (140), e57901, doi:10.3791/57901 (2018).

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