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Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
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Biochimie
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Journal JoVE Biochimie
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

12,117 Views

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10:27 min

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October 21, 2018

DOI:

10.3791/57901-v

DOI:
10.3791/57901-v

•

10:27 min
October 21, 2018

•

2 Views
, , , , ,
1Neutron Scattering Division,Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

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This protocol demonstrates how to obtain a low-resolution ab initio model and structural details of a detergent-solubilized membrane protein in solution using small-angle neutron scattering with contrast-matching of the detergent.

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Membrane ProteinSmall-angle Neutron ScatteringContrast MatchingDeuterium LabelingAb Initio ModelingOligomeric StateSizeShapeLow Resolution StructureDetergentScattering Length DensityContrast Match PointE. ColiDeuterated ProteinMinimal MediumBioreactor

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Cite this Article

Oliver, R. C., Naing, S., Weiss, K. L., Pingali, S. V., Lieberman, R. L., Urban, V. S. Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling. J. Vis. Exp. (140), e57901, doi:10.3791/57901 (2018).

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