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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
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Bioquímica
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JoVE Journal Bioquímica
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

9,403 Views

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11:08 min

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June 19, 2018

DOI:

10.3791/57862-v

DOI:
10.3791/57862-v

•

11:08 min
June 19, 2018

•

1 Views
, , , , , , , ,
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

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DNA RepairProofreadingMALDI-TOF Mass SpectrometrySingle Nucleotide ExtensionDNA FidelityDNA PolymerasePrimer-template PreparationMismatch SubstrateReaction BufferDdNTPsDNA Polymerase DilutionReaction TemperatureReaction Termination

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Su, K., Goodman, S. D., Lai, H., Yen, R., Hu, W., Cheng, W., Lin, L., Yang, Y., Fang, W. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J. Vis. Exp. (136), e57862, doi:10.3791/57862 (2018).

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