Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
9,432 Views
•
11:08 min
•
June 19, 2018
DOI:
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Summary
Automatically generated
A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.
Read Article
Cite this Article
Su, K., Goodman, S. D., Lai, H., Yen, R., Hu, W., Cheng, W., Lin, L., Yang, Y., Fang, W. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J. Vis. Exp. (136), e57862, doi:10.3791/57862 (2018).
Copy