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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

10,766 Views

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08:56 min

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July 27, 2018

DOI:

10.3791/57855-v

DOI:
10.3791/57855-v

•

08:56 min
July 27, 2018

•

1 Views
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1Texas A&M Agrilife Center at Dallas, 2Noble Research Center,Oklahoma State University, 3Department of Biology, College of Engineering and Natural Sciences,The University of Tulsa, 4Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology,Oklahoma State University

Summary

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Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.

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DNA AnalysisCrude Virion ExtractionRNA AnalysisInfected LeavesNew Virus GenomesEnvironmental VirologyBadnavirusesHomogenizationDifferential CentrifugationBio-analyzerLaboratory ProtocolLeaf GrindingUreaDetergentCentrifugationPellet SeparationVirus PurificationDNA Sequencing

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Verchot, J., Thapa, A., Wijayasekara, D., Hoyt, P. R. Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes. J. Vis. Exp. (137), e57855, doi:10.3791/57855 (2018).

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