/
/
Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Capítulos
- 00:04Título
- 00:432,4,6-Trinitrophenyl (TNP)-polysaccharide and TNP-keyhole Limpet Hemocyanin (KLH) Immunization and Serum Preparation
- 01:54Enzyme-linked Immunosorbent Analysis (ELISA)
- 04:17Results: Representative Thymus-independent (TI) and Thymus-dependent (TD) Ig Isotype responses to TNP-polysaccharide and TNP-KLH Immunization
- 05:36Conclusion
In this paper, we describe a protocol to characterize T-dependent and T-independent immunoglobulin (Ig) isotype responses in mice using ELISA. This method used alone or in combination with flow cytometry will allow researchers to identify differences in B cell-mediated Ig isotype responses in mice following T-dependent and T-independent antigen immunization.
