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Zuivering en reconstructie van TRPV1 spectroscopische p.a.
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Bioquímica
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JoVE Journal Bioquímica
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Zuivering en reconstructie van TRPV1 spectroscopische p.a.

7,827 Views

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11:53 min

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July 03, 2018

DOI:

10.3791/57796-v

DOI:
10.3791/57796-v

•

11:53 min
July 03, 2018

•

1 Views
, , , ,
1Department of Physiology,University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics,Vanderbilt University Medical Center, 3CuriRX, Inc.

Summary

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Dit artikel wordt beschreven specifieke methoden voor biochemische hoeveelheid wasmiddel-ontbindend TRPV1 spectroscopische p.a.. De gecombineerde protocollen bieden biochemische en biofysische hulpmiddelen die kunnen worden aangepast om de structurele en functionele studies voor zoogdieren ionenkanalen in een membraan-gecontroleerde omgeving.

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Cite this Article

Sierra-Valdez, F. J., Stein, R. A., Velissety, P., Vasquez, V., Cordero-Morales, J. F. Purification and Reconstitution of TRPV1 for Spectroscopic Analysis. J. Vis. Exp. (137), e57796, doi:10.3791/57796 (2018).

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