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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Biochemie
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JoVE Journal Biochemie
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

7,830 Views

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11:53 min

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July 03, 2018

DOI:

10.3791/57796-v

DOI:
10.3791/57796-v

•

11:53 min
July 03, 2018

•

1 Views
, , , ,
1Department of Physiology,University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics,Vanderbilt University Medical Center, 3CuriRX, Inc.

Summary

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This article describes specific methods to obtain biochemical quantities of detergent-solubilized TRPV1 for spectroscopic analysis. The combined protocols provide biochemical and biophysical tools that can be adapted to facilitate structural and functional studies for mammalian ion channels in a membrane-controlled environment.

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Cite this Article

Sierra-Valdez, F. J., Stein, R. A., Velissety, P., Vasquez, V., Cordero-Morales, J. F. Purification and Reconstitution of TRPV1 for Spectroscopic Analysis. J. Vis. Exp. (137), e57796, doi:10.3791/57796 (2018).

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