É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations
Capítulos
- 00:04Título
- 00:41Differentiation of Human Pluripotent Stem Cells (hPSC) to Retinal Pigment Epithelium (RPE) Cells and Isolation
- 03:02Whole-cell Patch Clamp RPE Cell Culture Preparation
- 04:42Results: Representative Mature RPE Cell Status Validation
- 05:28Conclusion
Here we present a protocol to differentiate retinal pigment epithelium (RPE) cells from human pluripotent stem cells bearing patient-derived mutations. The mutant cell lines may be used for functional analyses including immunoblotting, immunofluorescence, and patch clamp. This disease-in-a-dish approach circumvents the difficulty of obtaining native human RPE cells.
