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局部钙离子梯度对大泡囊膜重塑的响应

JoVE Journal
Química

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JoVE Journal Química

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

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局部钙离子梯度对大泡囊膜重塑的响应

DOI:

10.3791/57789-v

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08:15 min

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July 16, 2018

•

Baharan Ali Doosti, Ann-Sofie Cans, Gavin D.M. Jeffries, Tatsiana Lobovkina

¹Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Capítulos

00:04Título
00:41Preparation of Small Lipid Vesicles
02:38Preparation of GUV-MLV Complexes
04:12Micropipette Preparation and Microinjection
06:09Formation and Translation of Membrane Tubular Protrusions (MTPs) using the Calcium Ion Source
06:57Results: Formation of Membrane Tubular Protrusions in Giant Vesicles Upon Localized Application of Calcium Ion Gradients
07:37Conclusion

Summary

Tadução automática

我们提出了一种非接触微操作系统泡的技术, 利用局部钙离子梯度。在巨大的脂质囊的附近注射钙离子溶液, 用于重塑脂质膜, 导致膜管状突起的产生。

Tags

Keywords: Membrane Remodeling Giant Vesicles Calcium Ion Gradients Lipid Membrane Cell Membrane Reshaping GUV-MLV Small Lipid Vesicles Lipid Film Phosphate-buffered Saline Glycerol Sonication Vortex Mixing Vacuum Desiccation

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