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Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
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Chimie
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Journal JoVE Chimie
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

DOI:
10.3791/57789-v

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08:15 min

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July 16, 2018

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, , ,
1Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Chapitres

  • 00:04Titre
  • 00:41Preparation of Small Lipid Vesicles
  • 02:38Preparation of GUV-MLV Complexes
  • 04:12Micropipette Preparation and Microinjection
  • 06:09Formation and Translation of Membrane Tubular Protrusions (MTPs) using the Calcium Ion Source
  • 06:57Results: Formation of Membrane Tubular Protrusions in Giant Vesicles Upon Localized Application of Calcium Ion Gradients
  • 07:37Conclusion

Summary

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We present a technique for contactless micromanipulation of vesicles, using localized calcium ion gradients. Microinjection of a calcium ion solution, in the vicinity of a giant lipid vesicle, is utilized to remodel the lipid membrane, resulting in the production of membrane tubular protrusions.

Tags

Keywords: Membrane RemodelingGiant VesiclesCalcium Ion GradientsLipid MembraneCell Membrane ReshapingGUV-MLVSmall Lipid VesiclesLipid FilmPhosphate-buffered SalineGlycerolSonicationVortex MixingVacuum Desiccation

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