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Biologia do Desenvolvimento

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原发性细胞衰老的诱导与鉴定

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Induction and Validation of Cellular Senescence in Primary Human Cells

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原发性细胞衰老的诱导与鉴定

DOI:

10.3791/57782-v

•

08:18 min

•

June 20, 2018

•

Alejandra Hernandez-Segura, Simone Brandenburg, Marco Demaria

¹European Research Institute for the Biology of Aging,University of Groningen, University Medical Center Groningen

Capítulos

00:04Título
00:43Doxorubicin-induced Senescence
01:54Senescence-associated ß-galactosidase Staining
03:35EdU Incorporation Assay
06:30Results: Quantification of Senescence-associated Markers
07:17Conclusion

Summary

Tadução automática

在这里, 我们讨论了一系列的诱导和确认细胞衰老在培养细胞的协议。我们专注于不同的衰老诱发刺激, 并描述了常见的衰老相关标记的量化。我们提供的技术细节, 以成纤维细胞作为模型, 但协议可以适应各种细胞模型。

Tags

Cellular Senescence Primary Human Cells Induction Validation Beta-galactosidase Staining Doxorubicin Fibroblasts Aging Cancer Cell Culture Microscopy

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