/
/
Induction and Validation of Cellular Senescence in Primary Human Cells
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Induction and Validation of Cellular Senescence in Primary Human Cells
16,073 Views
•
08:18 min
•
June 20, 2018
DOI:
Summary
Automatically generated
Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.
Read Article
Cite this Article
Hernandez-Segura, A., Brandenburg, S., Demaria, M. Induction and Validation of Cellular Senescence in Primary Human Cells. J. Vis. Exp. (136), e57782, doi:10.3791/57782 (2018).
Copy