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Imunologia e Infecção
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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

DOI:
10.3791/57777-v

•

07:52 min

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September 10, 2018

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, , , ,
1BioMarin Pharmaceutical Inc

Capítulos

  • 00:04Título
  • 00:56Cell Plating and Sample Preparation
  • 03:43Sample Addition and Cell Viability Staining
  • 05:44Results: Representative Neutralizing Antibody (NAb) Detection by Enzyme Replacement Therapy (ERT)
  • 07:09Conclusion

Summary

Tadução automática

Tadução automática

Here we present a cell-based flow cytometry method to detect neutralizing antibodies or other factors that interfere with the cellular uptake of enzyme replacement therapies in a human matrix, such as cerebral spinal fluid (CSF) or human serum.

Tags

Keywords: AntibodiesNeutralizingEnzyme Replacement TherapyCell-based AssayImmunogenicityBioanalyticalDrug SafetyEfficacyPharmacokineticsPharmacodynamicsJurkat CellsMannose-6-phosphate ReceptorFlow CytometryERT BeadsMagnetic SeparationRotary Shaker

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