Journal
/
Neurociência
/
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

DOI:
10.3791/57723-v

•

10:08 min

•

June 08, 2018

•

, , ,
1Kavli Institute for Systems Neuroscience and Centre for Neural Computation,Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health

Capítulos

  • 00:04Título
  • 00:57Striatum, Hippocampus, and Cortex Harvest: Technique 1
  • 03:15Striatum, Hippocampus, and Cortex Harvest: Technique 2
  • 04:33Generation of Single Cell Suspensions
  • 06:01P0-2 Wild-Type (WT) Pup Transplantation
  • 08:13Results: Representative Grafted Interneuron Analyses
  • 09:28Conclusion

Summary

Tadução automática

Tadução automática

Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups.

Tags

Homochronic TransplantationInterneuron PrecursorsPostnatal Mouse BrainsDevelopmental NeuroscienceCell HarvestingCell GraftingStriatumHippocampusFluorescent Reporter MouseArtificial Cerebrospinal FluidDissecting MicroscopeForceps

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination

Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Simultaneous Cryosectioning of Multiple Rodent Brains

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

Read Article