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Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
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Neuroscienze
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JoVE Journal Neuroscienze
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

DOI:
10.3791/57723-v

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10:08 min

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June 08, 2018

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, , ,
1Kavli Institute for Systems Neuroscience and Centre for Neural Computation,Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health

Capitoli

  • 00:04Titolo
  • 00:57Striatum, Hippocampus, and Cortex Harvest: Technique 1
  • 03:15Striatum, Hippocampus, and Cortex Harvest: Technique 2
  • 04:33Generation of Single Cell Suspensions
  • 06:01P0-2 Wild-Type (WT) Pup Transplantation
  • 08:13Results: Representative Grafted Interneuron Analyses
  • 09:28Conclusion

Summary

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Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups.

Tags

Homochronic TransplantationInterneuron PrecursorsPostnatal Mouse BrainsDevelopmental NeuroscienceCell HarvestingCell GraftingStriatumHippocampusFluorescent Reporter MouseArtificial Cerebrospinal FluidDissecting MicroscopeForceps

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