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2 en 1: purificación de la afinidad para el análisis paralelo de proteínas y complejos de proteína-metabolito de un solo paso

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

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2 en 1: purificación de la afinidad para el análisis paralelo de proteínas y complejos de proteína-metabolito de un solo paso

10,855 Views

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08:23 min

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August 06, 2018

DOI:

10.3791/57720-v

DOI:

10.3791/57720-v

•

08:23 min

August 06, 2018

•

7 Views

Marcin Luzarowski*¹, Izabela Wojciechowska*¹, Aleksandra Skirycz

¹Max Planck Institute of Molecular Plant Physiology

Tags

Affinity Purification Protein-protein Complexes Protein-metabolite Complexes Parallel Analysis Basic Research Medical Pharmaceutical Biotechnological Small Molecular Ligands In Vivo Conditions Interacting Molecules PSBL Arabidopsis Thaliana Cell Culture MSMO Medium Logarithmic Growth Phase Mixer Mill Lysis Buffer IgG Sepharose Beads

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