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2 में 1: प्रोटीन और प्रोटीन-Metabolite परिसरों के समानांतर विश्लेषण के लिए एक कदम संबध शुद्धि

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

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2 में 1: प्रोटीन और प्रोटीन-Metabolite परिसरों के समानांतर विश्लेषण के लिए एक कदम संबध शुद्धि

10,855 Views

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08:23 min

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August 06, 2018

DOI:

10.3791/57720-v

DOI:

10.3791/57720-v

•

08:23 min

August 06, 2018

•

7 Views

Marcin Luzarowski*¹, Izabela Wojciechowska*¹, Aleksandra Skirycz

¹Max Planck Institute of Molecular Plant Physiology

Tags

Affinity Purification Protein-protein Complexes Protein-metabolite Complexes Parallel Analysis Basic Research Medical Pharmaceutical Biotechnological Small Molecular Ligands In Vivo Conditions Interacting Molecules PSBL Arabidopsis Thaliana Cell Culture MSMO Medium Logarithmic Growth Phase Mixer Mill Lysis Buffer IgG Sepharose Beads

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