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果蝇的口腔细菌感染和脱落
JoVE Journal
Biologia
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JoVE Journal Biologia
Oral Bacterial Infection and Shedding in Drosophila melanogaster
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果蝇的口腔细菌感染和脱落

DOI:
10.3791/57676-v

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09:32 min

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May 31, 2018

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, , , ,
1Institute of Evolutionary Biology, School of Biological Sciences,University of Edinburgh, 2IGDR – CNRS UMR 6290, 3Centre for Immunity, Infection and Evolution,University of Edinburgh

Capítulos

  • 00:05Título
  • 00:46Maintaining and Preparing the Flies
  • 02:52Bacterial Culture
  • 04:15Orally Infecting Flies
  • 06:18Measuring Bacterial Load and Bacterial Shedding
  • 07:18Results: P. aeruginosa and P. entomophila Infections
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

本议定书描述了用细菌病原体口服和感染果蝇果蝇 melanogaster 的方法, 并测定肠道感染后感染细菌的数量。进一步描述了免疫突变体对口腔细菌感染后蝇存活的影响。

Tags

Keywords: Oral Bacterial InfectionDrosophila MelanogasterGut ImmunocompetenceHost DefensePathogenMicrobiology ProtocolsLewis MediumPopulation CageApple AgarPBSP. EntomophilaP. AeruginosaLB Broth

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