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Oral Bacterial Infection and Shedding in Drosophila melanogaster
JoVE Journal
Biologia
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JoVE Journal Biologia
Oral Bacterial Infection and Shedding in Drosophila melanogaster

Oral Bacterial Infection and Shedding in Drosophila melanogaster

11,055 Views

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09:32 min

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May 31, 2018

DOI:

10.3791/57676-v

DOI:
10.3791/57676-v

•

09:32 min
May 31, 2018

•

10 Views
, , , ,
1Institute of Evolutionary Biology, School of Biological Sciences,University of Edinburgh, 2IGDR – CNRS UMR 6290, 3Centre for Immunity, Infection and Evolution,University of Edinburgh

Summary

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This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial infection.

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Cite this Article

Siva-Jothy, J. A., Prakash, A., Vasanthakrishnan, R. B., Monteith, K. M., Vale, P. F. Oral Bacterial Infection and Shedding in Drosophila melanogaster. J. Vis. Exp. (135), e57676, doi:10.3791/57676 (2018).

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