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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
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09:10 min
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May 22, 2018
DOI:
1Department of Neurobiology, F. M. Kirby Neurobiology Center,Boston Children’s Hospital, Harvard Medical School, 2Proteomic Mass Spectrometry,Wellcome Trust Sanger Institute, Wellcome Genome Campus, 3Centre for Molecular Informatics, Department of Chemistry,University of Cambridge, 4Functional Proteomics Group, Chester Beatty Laboratories,Institute of Cancer Research
Summary
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Here we present the proteogenomic tool PoGo and protocols for fast, quantitative, post-translational modification and variant enabled mapping of peptides identified through mass spectrometry onto reference genomes. This tool is of use to integrate and visualize proteogenomic and personal proteomic studies interfacing with orthogonal genomics data.
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Schlaffner, C. N., Pirklbauer, G. J., Bender, A., Steen, J. A., Choudhary, J. S. A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes. J. Vis. Exp. (135), e57633, doi:10.3791/57633 (2018).
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